Mouse kidney M430v2 Sex Balanced (Aug06) RMA

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Summary

The July 2006 Kidney QTL Consortium data set provides estimates of mRNA expression in the adult kidney of 70 genetically diverse strains of mice including 54 BXD recombinant inbred strains, a set of 15 inbred strains, and 1 F1 hybrid; D2B6F1. Kidney samples were processed using a total of 153 Affymetrix Mouse Expression 430 2.0 microarrays (M430v2.0). This particular data set was processed using the RMA protocol. CAUTION: This dataset is not sex-balanced.

Experiment design

Sample Processing: : Samples were processed at the Mount Sinai School of Medicine in the laboratory of Dr. Erwin Bottinger. All processing steps were performed Kremena Star. Total RNA was extracted with TRIZOL, followed by Rneasy Mini Kit purification (QUAGEN) by Kremena Star at MSSM. RNA purity was evaluated using the 260/280 nm (protein contamination) and the 260/230 nm (TRIZOL contamination) absorbance ratios, and values had to be greater than 1.9 and 0.8 respectively. Most of the samples 260/280 nm values fell between 1.9 and 2.1 and the majority of the 260/230 nm measurements were in the range 1.8 to 2.3. RNA integrity was assessed with the Agilent Bioanalyzer 2100. We set a quality threshold at 28s/18s rRNA greater than 0.9 or RNA integrity number (RIN) of greater than 6.9. When the two metrics were in disagreement we gave priority on the RIN value as recommended by the Agilent Bioanalyzer 2100 technical support representative. Synthesis of cDNA template from total RNA was performed using the one-cyclecDNA synthesis kit from Affymetrix (Affymetrix, P/N 900431). The Affymetrix IVT-labeling kit ((Affymetrix, P/N 900449)) was used to synthesize labeled cRNA. The cRNA was evaluated using both the 260/280 ratio (values of 1.9 to 2.1). The cRNA is then sheared using a fragmentation buffer included in the Affymetrix GeneChip Sample Cleanup (Affymetrix, P/N 900371). Fragmented cRNA samples were accessed with Agilent Bioanalyzer 2100 (cRNA frgments between 25 and 200 nucleotides are required for optimal hybridization and immediately injected onto the array. The arrays were hybridized and washed following standard Affymetrix protocols.

Replication and Sample Balance: We obtained a male sample pool and female sample pool from each isogenic group. Initially all strains were represented by male and female samples, however, not all data sets passed the quality control steps. Forty-two (thirty-one BXD, D2B6F1 and ten inbred strains) are represented by male and female samples.

Experimental Design and Batch Structure: The data set consists of arrays processed in twenty-three groups over a six month period (March 2006 to July 2006). Each group consists of 4 to 12 arrays. All arrays were processed using the Affymetrix Eukaryotic Sample and Array Processing protocol (701024 Rev. 3), by a single operator, Kremena Star. All samples in a group were labeled on the same. The hybridization station accommodates up to 4 samples, and since most of the groups had 12 samples, processed in 3 batches on the same day. Samples were washed in groups of four and kept at 4° C until all 12 (or 4-12) arrays were ready to scan. Samples were scanned in sets of four.

This table lists all arrays ordered by strain and includes Microarray ID, number of mice per pool, Microarray date, GAPDH 3`/5`Signal Ratio, Percent of transcripts present on the microarray chip, Strain, Generation, Sex, Age and Source of mice.
Microarray_ID # mice Microarray Date GAPDH (3`/5`) % present strains generation sex age Mice Source
GKHI-KS-050603.07-051706 3 05/14/02 0.73 37.6 C57BL/6J   M 56 UTM RW
GKHI-KS-070803.01-051706 3 05/14/02 0.75 41.6 C57BL/6J   F 69 UTM RW
GKHI-KS-DBA-Male-070706 2 07/06/02 0.71 37.3 DBA/2J   M 56 JAX
GKHI_KS_121404.75-042106 4 04/18/02     DBA/2J   F 59 UTM RW
GKHI_KS_121404.75-033006 4 03/19/02 0.73 48.5 DBA/2J   F 59 UTM RW
GKHI_KS_121404.78-042006 4 04/17/02 0.83 40.6 DBA/2J   F 59 UTM RW
GKHI_KS_070804.39-042006 4 04/17/02 0.82 39.0 D2B6F1   M 59 UTM RW
GKHI_KS_030904.01-042006 4 04/17/02 0.82 35.9 D2B6F1   M 57 UTM RW
GKHI-KS-121404.73-070706 2 07/06/02 0.76 36.3 D2B6F1   F 69 UTM RW
GKHI-KS-010705.38-051206 5 05/09/02 0.81 39.9 BXD1   M 59 Harvard/BIDMC
GKHI-KS-060905.19 5 06/05/02 0.75 42.3 BXD1   M 68 UTM RW
GKHI-KS-051206.13-070706 3 07/06/02 0.71 36.3 BXD1   F 57 UTM RW
GKHI-KS-021304.10-051206 4 05/09/02 0.81 39.1 BXD2   M 61 Harvard/BIDMC
GKHI-KS-040303-04-050406 3 05/01/02 0.80 37.6 BXD5   F 56 UMemphis
GKHI-KS-010705-53-050306 5 05/01/02 0.76 37.1 BXD5   F 58 Harvard/BIDMC
GKHI-KS-031103.01-062206 3 06/21/02 0.75 37.1 BXD5   M 71 UMemphis
GKHI-KS-040505-51-050306 5 05/01/02 0.71 35.5 BXD6   M 58 UTM RW
GKHI-KS-092705-29--050406 5 05/02/02 0.75 36.1 BXD6 160 F 64 UTM RW
GKHI_KS_092404.01-042106 4 04/18/02 0.71 36.3 BXD8   M 59 Harvard/BIDMC
GKHI-KS-051205-25-042706 5 04/23/02 0.92 37.9 BXD8   F 77 UTM RW
KS-021605-17-042606 5 04/22/02 0.85 40.8 BXD9   F 67 UTM RW
KS-032905-32-042606 5 04/22/02 0.91 36.8 BXD9   F 60 UTM RW
GKHI-KS-062006.08-070706 3 07/06/02 0.74 36.3 BXD9   M 78 UTM RW
GKHI-KS-031505.22-051206 5 05/09/02 0.74 39.5 BXD11   F 65 UTM RW
GKHI-KS-031605.01 5 06/05/02 0.74 43.4 BXD11   F 69 UTM RW
GKHI_KS_102104.40-042106 4 04/18/02 0.72 38.5 BXD12   M 60 Harvard/BIDMC
GKHI-KS-112002.07-051106 2 05/08/02 0.77 42.0 BXD12   F 64 UMemphis
GKHI-KS-120904.33-051206 4 05/09/02 0.71 38.4 BXD13   F 60 Harvard/BIDMC
GKHI-KS-042304.01 4 06/05/02 0.71 44.1 BXD13   F 58 Harvard/BIDMC
GKHI-KS-020905.34-051106 5 05/08/02 0.75 40.7 BXD14   F 68 UTM RW
GKHI-KS-022405.46-051106 5 05/08/02 0.71 40.2 BXD14   F 60 Harvard/BIDMC
GKHI-KS-091704.09-062206 4 06/21/02 0.73 39.0 BXD14   M 59 Harvard/BIDMC
GKHI-KS-013004.45-062206 4 06/21/02 0.76 38.5 BXD15   M 61 Harvard/BIDMC
GKHI-KS-022405.43-051106 5 05/08/02 0.73 40.5 BXD15   F 60 Harvard/BIDMC
GKHI-KS-041604.10-051106 4 05/08/02 0.73 42.6 BXD15   F 60 Harvard/BIDMC
GKHI-KS-031805.01-051106 5 05/08/02 0.79 42.4 BXD16   F 59 Harvard/BIDMC
GKHI-KS-031805.04-051106 5 05/08/02 0.77 39.9 BXD16   M 60 Harvard/BIDMC
GKHI-KS-040805.10-051006 5 05/05/02 0.93 38.7 BXD18   F 59 Harvard/BIDMC
GKHI-KS-052804.09-051106 4 05/05/02 0.67 37.6 BXD19   F 60 Harvard/BIDMC
GKHI-KS-010705.47-051106 5 05/05/02 0.73 42.3 BXD19   F 60 Harvard/BIDMC
GKHI-KS-010705.44-070706 3 07/06/02 0.73 36.1 BXD19   M 60 Harvard/BIDMC
GKHI-KS-062905.07-051106 5 05/05/02 0.72 37.9 BXD20   M 60 Harvard/BIDMC
GKHI-KS-072104.58-051106 4 05/05/02 0.73 37.0 BXD20   F 59 Harvard/BIDMC
GKHI-KS-050405.21-051206 4 05/09/02 0.81 35.1 BXD21   F 60 Harvard/BIDMC
GKHI-KS-040705.24 5 06/05/02 0.80 40.2 BXD21   F 99 UAB
GKHI-KS-110405.01-051006 5 05/05/02 0.71 42.1 BXD22   F 60 Harvard/BIDMC
GKHI-KS-110405.04-051006 5 05/05/02 0.76 40.6 BXD22   M 60 Harvard/BIDMC
GKHI-KS-040805.01-051206 5 05/09/02 0.74 36.4 BXD23   F 60 Harvard/BIDMC
GKHI-KS-040805.04-051006 5 05/05/02 0.73 39.3 BXD23   M 60 Harvard/BIDMC
GKHI_KS_091704.13-042106 4 04/18/02 0.73 37.7 BXD24   M 59 Harvard/BIDMC
GKHI-KS-040303-20-050206 3 04/30/02 0.77 36.3 BXD24   F 71 UMemphis
GKHI-KS-021805.20-051006 5 05/05/02 0.83 40.5 BXD25   F 67 UAB
GKHI-KS-090705.05-062206 5 06/21/02 0.76 38.4 BXD25   M 58 UTM RW
GKHI-KS-090705.03-051006 5 05/05/02 0.81 40.1 BXD25   F 58 UTM RW
GKHI-KS-022105.42-051006 5 05/05/02 0.81 41.8 BXD27   M 70 UAB
GKHI-KS-032205.31-051006 5 05/05/02 0.74 39.0 BXD27   M 60 UTM RW
GKHI-KS-060706.10-070706 3 07/06/02 0.69 37.8 BXD27   F 85 UTM RW
GKHI-KS-012805-41-050506 5 05/04/02 0.81 34.2 BXD28   F 60 Harvard/BIDMC
GKHI-KS-012805-44-050506 5 05/04/02 0.81 35.8 BXD28   M 60 Harvard/BIDMC
GKHI-KS-012805-38-050506 5 05/04/02 0.77 42.7 BXD29   F 60 Harvard/BIDMC
GKHI-KS-012805-35-050506 5 05/04/02 0.84 35.8 BXD29   F 60 Harvard/BIDMC
GKHI-KS-012805-32-050506 5 05/04/02 0.74 39.9 BXD31   F 60 Harvard/BIDMC
GKHI-KS-100604-07-050506 4 05/04/02 0.79 40.2 BXD31   M 60 Harvard/BIDMC
GKHI-KS-021605.26-051706 5 05/14/02 1.11 39.0 BXD32   F 63 UTM RW
GKHI-KS-112002.01-051206 2 05/09/02 0.76 38.1 BXD32   F 60 UMemphis
GKHI-KS-072605-01-050506 5 05/04/02 0.79 41.3 BXD33 50 F 63 UTM RW
GKHI-KS-091405-23-050506 5 05/04/02 0.84 38.4 BXD33 50 M 76 UTM RW
GKHI-KS-111104-18-050506 4 05/04/02 0.79 42.2 BXD36   F 61 Harvard/BIDMC
GKHI-KS-031804-07-050506 4 05/04/02 0.85 42.5 BXD36   F 58 Harvard/BIDMC
GKHI-KS-092005.16-051206 5 05/09/02 0.72 37.2 BXD38 46 F 65 UTM RW
GKHI-KS-031403.01-060806 1 06/07/02 0.69 39.6 BXD38   M 69 UMemphis
GKHI-KS-090104.21-051106 4 05/08/02 0.72 36.3 BXD39   F 60 Harvard/BIDMC
GKHI-KS-040204.30 4 06/05/02 0.77 43.9 BXD39   F 59 Harvard/BIDMC
GKHI-KS-051805-16-050506 5 05/04/02 2.16 43.8 BXD40   F 61 UTM RW
GKHI-KS-111902-04-050506 2 05/04/02 0.84 41.0 BXD40   F 56 UMemphis
GKHI-KS-050604-01-050406 4 05/01/02 0.85 35.6 BXD43 23 F 61 UTM RW
GKHI-KS-080905-43-050406 5 05/01/02 0.78 33.7 BXD43 28 F 62 UTM RW
GKHI-KS-031004-01-050406 4 05/01/02 0.75 36.2 BXD44 21 F 57 UTM RW
GKHI-KS-020504-01-050406 4 05/01/02 0.75 30.8 BXD44 20 M 66 UTM RW
GKHI-KS-071504-01-050406 4 05/01/02 0.74 37.7 BXD45 20 F 58 UTM RW
GKHI-KS-081104-05-050406 4 05/01/02 0.78 34.2 BXD45 20 M 93 UTM RW
GKHI-KS-031204-01-050406 4 05/01/02 0.75 35.6 BXD48 22 M 60 UTM RW
GKHI-KS-021104.06-051706 4 05/14/02 0.75 41.1 BXD48 21 F 58 UTM RW
GKHI-KS-033005-21-050306 5 05/01/02 0.78 34.9 BXD51 27 M 64 UTM RW
GKHI-KS-090204-01-050306 4 05/01/02 0.72 38.3 BXD51 24 F 63 UTM RW
GKHI_KS-010704.01-040606 4 04/03/02 0.75 37.5 BXD60 21 M 64 UTM RW
GKHI_KS_013004.38-042006 4 04/17/02 0.78 40.1 BXD60 21 F 60 UTM RW
GKHI-KS-030905-28-050206 5 04/30/02 0.73 36.6 BXD61 20 F 63 UTM RW
GKHI-KS-050305-18-050206 5 04/30/02 0.83 35.8 BXD61 21 F 70 UTM RW
GKHI_KS-121803.01-040706 3 04/03/02 0.80 40.0 BXD62 20 M 54 UTM RW
GKHI_KS_021204.01-042006 4 04/17/02 0.85 39.8 BXD62 21 F 59 UTM RW
GKHI-KS-020905-25-050206 5 04/30/02 0.84 35.2 BXD63 21 M 70 UTM RW
GKHI-KS-040705.49-060806 2 06/07/02 0.85 39.4 BXD65 20 F 55 UTM RW
GKHI-KS-040406.12-060806 2 06/07/02 0.73 40.4 BXD65 23 F 60 UTM RW
GKHI-KS-052405-36-050406 5 05/02/02 0.84 36.8 BXD67 20 F 65 UTM RW
GKHI-KS-041205-01-050206 5 04/30/02 0.77 39.7 BXD67 20 F 54 UTM RW
GKHI-KS-062305-01-050206 5 04/30/02 0.76 36.0 BXD68 20 F 59 UTM RW
GKHI-KS-062305-09-050206 5 04/30/02 0.93 37.4 BXD68 20 F 64 UTM RW
GKHI_KS_110105.30-042006 5 04/17/02 0.80 39.0 BXD69 26 F 66 UTM RW
GKHI-KS-061504.64-062206 4 06/21/02 0.72 39.3 BXD69 20 M 55 UTM RW
GKHI_KS-050404.04-040606 4 04/03/02 0.77 38.3 BXD69 20 F 63 UTM RW
GKHI-KS-042705-01-042706 5 04/23/02 0.83 38.6 BXD70 21 F 64 UTM RW
GKHI-KS-051705-59-042706 5 04/23/02 0.89 38.5 BXD70 22 F 61 UTM RW
GKHI-KS-030805-40-042706 5 04/23/02 0.86 38.5 BXD73 23 F 61 UTM RW
GKHI-KS-041905.172-062206 5 06/21/02 0.79 37.6 BXD73 24 M 64 UTM RW
GKHI-KS-072605-03-042706 5 04/23/02 0.87 38.2 BXD73 25 F 72 UTM RW
GKHI-KS-041205-04-050406 5 05/02/02 0.83 41.4 BXD75 22 F 60 UTM RW
GKHI-KS-041205.07 5 06/05/02 0.79 45.8 BXD75 22 F 60 UTM RW
GKHI-KS-101805-35-050406 5 05/02/02 0.79 38.4 BXD77 24 F 60 UTM RW
GKHI-KS-070605.43 5 06/05/02 0.77 42.8 BXD77 23 F 62 UTM RW
GKHI-KS-071205-31-042706 5 04/23/02 0.91 37.7 BXD80 20 F 65 UTM RW
GKHI-KS-071205-2-042706 5 04/23/02 0.90 38.1 BXD80 20 F 65 UTM RW
KS-011305-11-042606 5 04/22/02 0.82 39.0 BXD85 22 M 91 UTM RW
KS-110805-27-042606 5 04/22/02 0.91 35.2 BXD85 25 F 63 UTM RW
KS-080404-28-042606 4 04/22/02 0.84 36.8 BXD86 21 F 58 UTM RW
KS-080504-04-042606 4 04/22/02 0.81 33.4 BXD86 20 M 77 UTM RW
KS-051705-57-042606 5 04/22/02 1.11 35.6 BXD87 20 M 63 UTM RW
KS-032905-46-042606 5 04/22/02 0.85 36.1 BXD87 20 M 57 UTM RW
GKHI-KS-080905-49-042706 5 04/23/02 0.89 37.6 BXD90 23 F 71 UTM RW
GKHI-KS-101105.26 5 06/05/02 0.84 43.6 BXD90 25 F 70 UTM RW
GKHI_KS-062304.02-040606 4 04/03/02 0.85 40.9 BXD92 21 M 55 UTM RW
GKHI_KS_071404.01-042006 4 04/17/02 0.77 39.5 BXD92 21 F 62 UTM RW
GKHI_KS_031005.17-042006 5 04/17/02 0.77 38.1 BXD96 20 M 65 UTM RW
GKHI-KS-111505.12 5 06/05/02 0.77 44.1 BXD96 23 M 66 UTM RW
GKHI-KS-012406.21-060806 3 06/07/02 0.73 36.6 BTBR T+tf/J   F 60 Harvard/BIDMC
GKHI-KS-012406.24-060806 3 06/07/02 1.28 39.2 BTBR T+tf/J   F 60 Harvard/BIDMC
GKHI-KS-030206.13-060806 3 06/07/02 0.71 41.2 BXSB/MpJ   F 61 Harvard/BIDMC
GKHI-KS-030206.16-060806 3 06/07/02 0.66 37.2 BXSB/MpJ   F 61 Harvard/BIDMC
GKHI-KS-011906.31-060806 3 06/07/02 0.80 37.2 C3H/HeJ   M 60 Harvard/BIDMC
GKHI-KS-011906.34-060806 3 06/07/02 0.76 38.5 C3H/HeJ   M 60 Harvard/BIDMC
GKHI-KS-060806.04-070706 3 07/06/02 0.68 37.3 C3H/HeJ   F 76 Harvard/BIDMC
GKHI-KS-071505.08-060806 3 06/07/02 0.71 37.4 C57BL/6ByJ   F 51 JAX
GKHI-KS-071505.11-060806 2 06/07/02 0.73 39.2 C57BL/6ByJ   F 51 JAX
GKHI-KS-030305.15-060806 3 06/07/02 0.71 37.4 CAST/Ei   F 64 JAX
GKHI-KS-031005.35-060906 3 06/08/02 0.70 35.7 CAST/Ei   M 64 JAX
GKHI-KS-022206.16-060906 3 06/08/02 0.71 37.9 KK/HlJ   F 61 Harvard/BIDMC
GKHI-KS-022206.07-060906 3 06/08/02 0.72 35.5 KK/HlJ   M 61 Harvard/BIDMC
GKHI-KS-031606.01-060906 3 06/08/02 0.86 35.9 MOLF/Ei   M 60 Harvard/BIDMC
GKHI-KS-022206.16-060906 3 06/08/02 0.87 37.4 MOLF/Ei   F 60 Harvard/BIDMC
GKHI-KS-012006.25-060906 3 06/08/02 0.75 38.3 NOD/LtJ   F 58 Harvard/BIDMC
GKHI-KS-012006.28-061306 3 06/11/02 0.82 37.1 NOD/LtJ   F 58 Harvard/BIDMC
GKHI-KS-032306.04.060906 3 06/08/02 0.73 40.2 NZB/BlNJ   F 61 Harvard/BIDMC
GKHI-KS-032306.07-060906 3 06/08/02 0.74 39.4 NZB/BlNJ   F 61 Harvard/BIDMC
GKHI-KS-020706.04-060906 3 06/08/02 0.71 41.6 NZW/LacJ   F 65 Harvard/BIDMC
GKHI-KS-020206.19-060906 3 06/08/02 0.77 36.7 NZW/LacJ   M 60 Harvard/BIDMC
GKHI-KS-012406.33-061306 3 06/11/02 0.95 35.3 PWD/PhJ   F 60 Harvard/BIDMC
GKHI-KS-012406.30-062006 3 06/18/02 0.88 36.3 PWD/PhJ   M 60 Harvard/BIDMC
GKHI-KS-020206.01-062206 3 06/21/02 1.02 35.9 PWK/PhJ   F 60 Harvard/BIDMC
GKHI-KS-020206.04-062206 3 06/21/02 0.96 38.7 PWK/PhJ   F 60 Harvard/BIDMC
GKHI-KS-020206.07-062206 3 06/21/02 0.98 36.6 PWK/PhJ   M 60 Harvard/BIDMC
GKHI-KS-020206.10-062206 3 06/21/02 0.87 35.2 PWK/PhJ   M 60 Harvard/BIDMC
GKHI-KS-052705.01-061306 2 06/11/02 0.72 38.3 WSB/EiJ   F 52 UTM RW
GKHI-KS-051005.07-061306 3 06/11/02 0.77 38.0 WSB/EiJ   M 58 JAX

About cases

The BXD genetic reference panel of recombinant inbred strains consists of just over 80 strains. The BXDs in this data set include 30 of the BXD strains made by Benjamin Taylor at the Jackson Laboratory in the 1970s and 1990s (BXD1 through BXD42). All of these strains are fully inbred. We have also included 24 BXD lines generated by Lu and Peirce. All of these strains have been genotyped at 13,377 SNPs.

Mouse Diversity Panel (MDP). We have profiled a MDP consisting 15 inbred strains and a pair of F1 hybrids; D2B6F1. These strains were selected for the following reasons: This panel will be a powerful tool in systems genetic analysis of a wide variety of traits, and will provide additional power in fine mapping modulators through an association analysis of sequence variants.

  • genetic and phenotypic diversity, including use by the Phenome Project
  • their use in making genetic reference populations including recombinant inbred strains, cosomic strains, congenic and recombinant congenic strains
  • their use by the Complex Trait Consortium to make the Collaborative Cross (Nairobi/Wellcome, Oak Ridge/DOE, and Perth/UWA)
  • genome sequence data from three sources (NHGRI, Celera, and Perlegen-NIEHS)
  • availability from The Jackson Laboratory
  1. BTBR T+tf/J
        Multiple recessive stock; Homozygotes show repeated waves of hair loss and regrowth, which begin in the nose and pass posteriorly along the body.
  2. C3H/HeJ
        Sequenced by Perlegen/NIEHS; paternal parent of the BXH panel; Phenome Project A list
  3. C57BL/6J
        Sequenced by NHGRI; parental strain of AXB/BXA, BXD, and BXH; Phenome Project A list
  4. C57BL/6ByJ
        Paternal substrain of B6 used to generate the CXB panel
  5. CAST/Ei
        Collaborative Cross strain sequenced by NIEHS; Phenome Project A list
  6. DBA/2J
        Sequenced by Perlegen/NIEHS and Celera; paternal parent of the BXD panel; Phenome Project A list
  7. KK/HlJ
        Sequenced by Perlegen/NIEHS
  8. NOD/LtJ
        Collaborative Cross strain sequenced by NIEHS; Phenome Project B list; diabetic
  9. PWD/PhJ
        Sequenced by Perlegen/NIEHS; parental strain for a consomic set by Forjet and colleagues
  10. PWK/PhJ
        Collaborative Cross strain; Phenome Project D list
  11. WSB/EiJ
        Collaborative Cross strain sequenced by NIEHS; Phenome Project C list
  12. D2B6F1
    F1 hybrid generated by crossing C57BL/6J with DBA/2J

These inbred strains can be ordered from The Jackson Laboratory. BXD43 through BXD100 are available from Lu Lu and colleagues at UTHSC.P>

About tissue

BXD animals were obtained from UTHSC, and the Jackson Laboratory (Table 1). Animals were housed at UTHSC, at Harvard/BIDMC (Glenn Rosen), at the University of Memphis (Douglas Matthews), or the Jackson Laboratory before sacrifice (see Table 1). Mice were killed by cervical dislocation and kidneys were removed and placed in RNAlater prior to dissection. Kidneys were dissected whole and cleaned from the adrenal glands by Hong Tao Zhang in Dr. Lu’s lab. Kidneys from two to six animals per strain were pooled and shipped to Kremena Star at Mount Sinai School of Medicine (MSSM). Animals used in this study were between 50 and 99 days of age (average of 62 days; see Table 1).

About platform

Affymetrix Mouse Genome 430 2.0 array: The 430v2 array consists of 992936 useful 25-nucleotide probes that estimate the expression of approximately 39,000 transcripts and the majority of known genes and expressed sequence tags. The array sequences were selected late in 2002 using Unigene Build 107 by Affymetrix. The UTHSC group has recently reannotated all probe sets on this array, producing more accurate data on probe and probe set targets. All probes were aligned to the most recent assembly of the Mouse Genome (Build 34, mm6) using Jim Kent's BLAT program. Many of the probe sets have been manually curated by Jing Gu and Rob Williams.

About data processing

Probe set data from the CHP file: The expression values were generated using RMA. The same simple steps described above were also applied to these values.

Probe level QC: Log2 probe data of all arrays were inspected in DataDesk. Inspection involved examining scatterplots of pairs of arrays for signal homogeneity (i.e., high correlation and linearity of the bivariate plots) and looking at all pairs of correlation coefficients.

Validation of strains and sex of each array data set: A subset of probes and probe sets with a Mendelian pattern of inheritance were used to construct a expression correlation matrix for all arrays and the ideal Mendelian expectation for each strain constructed from the genotypes. There should naturally be a very high correlation in the expression patterns of transcripts with Mendelian phenotypes within each strain, as well as with the genotype strain distribution pattern of markers for the strain.

Sex of the samples was validated using sex-specific probe sets such as Xist and Dby.

All data links (right-most column above) will be made active as sooon as the global analysis of these data by the Consoritum has been accepted for publication. Please see text on Data Sharing Policies, and Conditions and Limitations, and Contacts. Following publication, download a summary text file or Excel file of the PDNN probe set data. Contact RW Williams regarding data access probelms.

Acknowledgment

Data were generated with funds provided by a variety of public and private source to members of the Kidney Consortium. Members of the Kidney Consortium thank the following sources for financial support of this effort:

  • Erwin Bottinger, M.D.
    Grant Support: R01 DK60043-04
  • Russell Chesney, M.D.
    Grant Support: Le Bonheur Chair of Excellence in Pediatrics II
  • Lu Lu, M.D.
    Grant Support: NIH U01AA13499, U24AA13513
  • Peter Mundel
    Grant Support: NIH R01DK57683, NIH R01 DK 62472
  • Paul Klotman
    Grant Support: PO1 DK56492, PO1 DK56492.
  • Matthew Breyer
    Grant Support: DK-38226
  • Kenneth F. Manly, Ph.D.
    Grant Support: NIH P20MH062009 and U01CA105417
  • Robert W. Williams, Ph.D.
    Grant Support: NIH U01AA013499, P20MH062009, U01AA013499, U01AA013513

 

Notes

This text file originally generated by Kremena Star on July 22 ,2006. Updated by KS on July 25, 2006.