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Summary
This is a microRNA data set generated using the Affymetrix Genechip miRNA 4.0 array (see http://media.affymetrix.com/support/technical/datasheets/miRNA_4-0_and_4-1_datasheet.pdf).
The data set provides 3222 estimates of expression (mainly for mi-RNAs) for 45 retinas from C57BL/6J (B6 cases B6.01 to B6.17), DBA/2J (cases D2.01 to D2.16), and DBA/2J with a wildtype Gpnmb allele (D2GP.01 to D2GP.12). Cases range in age from 30 to 576 days and include both sexes.
We are using this data set in combination with the conventional Mouse Gene 1.0ST array to study normal and glaucomatous changes in gene expression as a function of age.
Please contact Lu Lu (lulu@uthsc.edu), Robert W. Williams (rwilliams@uthsc.edu), or Junming Yue (jyue@uthsc.edu).
Supported in part by Research to Prevent Blindness.
Experiment design
Index Sample ID Case ID Strain Sex DOB Sac date Age group Age (days) Age (month) EarTag Number Tube ID Agilent RNA concentration (ng/ul) RIN Nanodrop RNA concentration (ng/ul) 260/280 260/230 Batch ID Notes 1 B6.01 071515.08 C57BL/6J M 06/15/15 07/15/15 1~2 30 1.0 R11 173 10.00 181.22 2.09 2.12 2 best 2 B6.02 071515.09 C57BL/6J M 06/15/15 07/15/15 1~2 30 1.0 R12 121 N/A 148.52 2.10 1.69 2 best 3 B6.03 052314.01 C57BL/6J F 03/23/14 05/23/14 2~4 61 2.0 Ret 32 68 10.0 48.53 2.02 1.93 1 4 B6.04 062514.01 C57BL/6J M 04/10/14 06/25/14 2~4 76 2.5 Ret 21 81 9.0 168.7 2.06 1.97 1 5 B6.05 040816.03 C57BL/6J M 02/04/16 04/08/16 2~4 64 2.1 R74 385 10.00 448.16 1.98 2.12 2 6 B6.06 040816.04 C57BL/6J M 02/04/16 04/08/16 2~4 64 2.1 R75 226 8.70 167.31 2.04 2.13 2 7 B6.07 041014.19 C57BL/6J F 10/20/13 04/10/14 5~7 172 5.7 Ret 10 22 10.0 36.97 2.22 0.051 1 8 B6.08 101813.27 C57BL/6J M 05/06/13 10/18/13 5~7 165 5.5 Ret 7 38 10.0 54.47 2.08 0.094 1 9 B6.09 050214.03 C57BL/6J F 08/14/13 05/02/14 7~9 261 8.7 Ret 27 20 10.0 54.45 2.11 1.14 1 10 B6.10 041014.24 C57BL/6J M 09/02/13 04/10/14 7~9 220 7.3 Ret 12 38 9.50 58.97 2.08 2.14 1 11 B6.11 101813.17 C57BL/6J F 12/07/12 10/18/13 10~12 315 10.5 Ret 2 59 9.40 83.86 2.17 1.87 1 12 B6.12 052116.01 C57BL/6J M 05/19/15 05/22/16 10~12 369 12.3 R102 293 8.30 179.69 2.06 2.11 2 13 B6.13 052116.02 C57BL/6J M 05/19/15 05/22/16 10~12 369 12.3 R103 255 8.40 194.24 2.04 1.82 2 14 B6.14 042114.19 C57BL/6J F 03/11/13 04/21/14 13~15 406 13.5 1252 Ret 26 28 10.0 39.95 2.22 1.71 1 15 B6.15 050214.07 C57BL/6J M 03/20/13 05/02/14 13~15 408 13.6 Ret 28 30 10.0 51.81 2.06 2.07 1 16 B6.16 041714.02 C57BL/6J M 09/18/12 04/17/14 19~21 576 19.2 1117 Ret 17 25 10.0 51.55 2.13 1.13 1 17 B6.17 041714.03 C57BL/6J M 09/18/12 04/17/14 19~21 576 19.2 1116 Ret 18 28 10.0 65.11 2.01 2.16 1 18 D2.01 030116.02 DBA/2J M 01/29/16 03/01/16 1~2 32 1.1 R63 107 8.80 119.02 2.05 1.79 2 19 D2.02 030116.04 DBA/2J M 01/29/16 03/01/16 1~2 32 1.1 R65 202 9.10 250.38 2.03 1.67 2 20 D2.03 062714.02 DBA/2J F 03/23/14 06/27/14 2~4 104 3.5 Ret 20a 189.3 1.95 1.81 1 No Agilent as of 6/27/2014 21 D2.04 062714.01 DBA/2J M 03/19/14 06/27/14 2~4 100 3.3 Ret 19a 154 2.05 1.89 1 No Agilent as of 6/27/2014 22 D2.05 032316.03 DBA/2J M 01/23/16 03/23/16 2~4 60 2.0 R70 99 9.30 99.17 2.04 1.63 2 23 D2.06 032316.04 DBA/2J M 01/23/16 03/23/16 2~4 60 2.0 R71 205 9.10 124.33 2.03 1.24 2 24 D2.07 050214.11 DBA/2J F 12/07/13 05/02/14 5~7 146 4.9 Ret 29 100 10.0 86.47 2.14 1.9 1 25 D2.08 101813.08 DBA/2J M 03/21/13 10/18/13 5~7 211 7.0 Ret 1 52 9.9 86.98 2.11 2.12 1 26 D2.09 041014.23 DBA/2J F 08/24/13 04/10/14 7~9 229 7.6 Ret 11 21 9.9 44.39 2.17 1.15 1 27 D2.10 050214.15 DBA/2J F 07/27/13 05/02/14 7~9 279 9.3 Ret 30 37 10.0 43.57 2.13 2.04 1 28 D2.11 101813.23 DBA/2J F 11/29/12 10/18/13 10~12 323 10.8 Ret 6 77 9.7 105.02 2.06 2.14 1 29 D2.12 101813.19 DBA/2J M 12/08/12 10/18/13 10~12 314 10.5 Ret 3 46 9.9 65.7 2.13 1.71 1 30 D2.13 042114.18 DBA/2J F 03/19/13 04/21/14 13~15 398 13.3 1148 Ret 25 26 9.9 65.53 2.17 2.43 1 31 D2.14 042114.17 DBA/2J M 03/19/13 04/21/14 13~15 398 13.3 1130 Ret 24 40 9.4 36.98 2.07 0.8 1 32 D2.15 041014.06 DBA/2J M 9/6/2012 04/10/14 19~21 581 19.4 1123 Ret 8 35 9.9 52.99 2.12 0.091 1 33 D2.16 041014.07 DBA/2J F 9/6/2012 04/10/14 19~21 581 19.4 1124 Ret 9 30 9.5 52.44 2.19 0.08 1 34 D2GP.01 041416.15 DBA-Gpmnb(wp) F 03/15/16 04/14/16 1~2 30 1.0 R92 334 9.10 135.79 1.87 2.23 2 35 D2GP.02 120715.02 DBA-Gpmnb(wp) M 11/05/15 12/07/15 1~2 32 1.1 R31 85 9.20 106.06 2.04 2.06 2 36 D2GP.03 041416.04 DBA-Gpmnb(wp) F 02/02/16 04/14/16 2~4 72 2.4 R81 112 9.40 94.79 1.94 2.19 2 37 D2GP.04 120815.04 DBA-Gpmnb(wp) M 10/08/15 12/08/15 2~4 61 2.0 R39 89 N/A 118.84 2.05 1.97 2 38 D2GP.05 101813.21 DBA-Gpmnb(wp) F 04/09/13 10/18/13 5~7 192 6.4 Ret 4 53 9.9 74.13 2.14 2.28 1 39 D2GP.06 101813.22 DBA-Gpmnb(wp) M 04/09/13 10/18/13 5~7 192 6.4 Ret 5 54 10.0 81.32 2.1 2.19 1 40 D2GP.07 041014.27 DBA-Gpmnb(wp) F 08/29/13 04/10/14 7~9 224 7.5 Ret 15 14 10.0 37.03 2.06 1.44 1 41 D2GP.08 041014.28 DBA-Gpmnb(wp) M 08/29/13 04/10/14 7~9 224 7.5 Ret 16 29 9.9 63.83 2.03 2.08 1 42 D2GP.09 041014.25 DBA-Gpmnb(wp) F 06/12/13 04/10/14 10~12 302 10.1 Ret 13 31 9.8 60.73 2.07 2.12 1 43 D2GP.10 041014.26 DBA-Gpmnb(wp) M 06/12/13 04/10/14 10~12 302 10.1 Ret 14 24 10.0 65.1 2.06 1.09 1 44 D2GP.11 042114.08 DBA-Gpmnb(wp) F 01/14/13 04/21/14 13~15 462 15.4 1021 Ret 22 87 9.0 150.69 2.09 2.02 1 45 D2GP.12 042114.09 DBA-Gpmnb(wp) F 01/14/13 04/21/14 13~15 462 15.4 1022 Ret 23 18 10.0 44.16 2.11 0.069 1